8/4/2023 0 Comments Rochard wolf![]() ![]() Novel protein-protein interaction between Escherichia coli SoxS and the DNA binding determinant of the RNA polymerase α subunit: SoxS functions as a co-sigma factor and redeploys RNA polymerase from UP-element-containing promoters to SoxS-dependent promoters during oxidative stress. Genetic evidence for pre-recruitment as the mechanism of transcription activation by SoxS of Escherichia coli: the dominance of DNA binding mutations of SoxS. Characterization of TetD as a transcriptional activator of a subset of genes of the Escherichia coli SoxS/Mar/Rob regulons. ![]() Inhibition of Lon-dependent degradation of the Escherichia coli transcription activator SoxS by interaction with “soxbox” DNA or RNA polymerase. Sequence requirements for Lon-dependent degradation of the Escherichia colitranscription activator SoxS: identification of the SoxS residues critical to proteolysis and specific inhibition of in vitrodegradation by a peptide comprised of the N-terminal 21 amino acid residues. Two functions of the C-terminal domain of Escherichia coliRob: mediating “sequestration-dispersal” as a novel off-on switch for regulating Rob’s activity as a transcription activator and preventing degradation of Rob by Lon protease. Protein-protein interactions between sigma 70 region 4 of RNA polymerase and Escherichia coli SoxS, a transcription activator that functions by the prerecruitment mechanism: evidence for “off-DNA” and “on-DNA” interactions”. *These authors contributed equally to this work. Transcription activation by Escherichia coli Rob at class II promoters: protein-protein interactions between Rob’s N-terminal domain and the sigma 70 subunit of RNA polymerase. Taliaferro, L.P.*, Keen* III, E.F., Alberola-Sanchez, N. The program, whose courses are taught exclusively in the evening at UMBC’s Tech Center, started in fall, 2007 with 14 students in the MPS program and 3 more in the Certificate program. I am the Graduate Program Director and we expect that this MPS program will be followed by others at UMBC. The purpose of the program is to provide the means for working professionals in the biotechnology industry to expand their knowledge of the sciences underlying biotechnology and to learn good manufacturing practices, regulatory issues in biotechnology, as well as the tools of financial management, leadership and team building. In 2006-2007, I led the development of the curriculum for a Masters in Professional Studies: Biotechnology and a Certificate in Biotechnology Management. We also studied GRDR of zwf, which encodes glucose-6-phosphate dehydrogenase. Formation of a long-range secondary structure in the mRNA between the RBS and the ICS plays a key role in GRDR of gnd. We discovered that GRDR of gnd is exerted at the level of translation initiation and involves the “internal complementary sequence” (ICS), a regulatory element lying deep within the gnd mRNA and whose sequence is complementary to the ribosome binding site (RBS) of the mRNA. The main gene we studied was gnd, which encodes 6-phosphogluconate dehydrogenase, an enzyme of the pentose phosphate pathway. This form of regulation coordinates gene expression with the cellular growth rate as it is determined by the nutritional quality of the growth medium. During my initial years at UMBC, I worked on understanding growth rate-dependent regulation (GRDR) of central metabolism genes in E. I get great pleasure from all of them. In research, I have long studied mechanisms of gene regulation in bacteria. Like most faculty, my professional interests fall into three areas: research, teaching, and service. in Psychology, University of Cincinnati, 1963 Professional Interests in Microbiology, University of Cincinnati, 1968ī.A. in Microbiology, University of Cincinnati, 1970 In Molecular Genetics, Harvard Medical School, 1975
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |